t vaginalis strain g3  (ATCC)

Journal: IJID Regions

Article Title: Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021

doi: 10.1016/j.ijregi.2023.02.007

Figure Lengend Snippet: Characteristics of 119 women screened for Trichomonas vaginalis , HPV and Candida albicans .

Article Snippet: Mid-log phase cultures of MTZ-susceptible T. vaginalis strain G3 (ATCC, PRA-98D) were used as a control, counted using a disposable C-CHIP hemocytometer (DHC-B02, NanoEntek, Korea) and diluted with modified TYI medium [15] at 3.3 × 10 4 cells/mL (anaerobic condition) or 13.3 × 10 4 cells/mL (aerobic condition).

Techniques: Infection

Journal: IJID Regions

Article Title: Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021

doi: 10.1016/j.ijregi.2023.02.007

Figure Lengend Snippet: The value of minimal lethal concentration for five clinical isolates and reference strain G3.

Article Snippet: Mid-log phase cultures of MTZ-susceptible T. vaginalis strain G3 (ATCC, PRA-98D) were used as a control, counted using a disposable C-CHIP hemocytometer (DHC-B02, NanoEntek, Korea) and diluted with modified TYI medium [15] at 3.3 × 10 4 cells/mL (anaerobic condition) or 13.3 × 10 4 cells/mL (aerobic condition).

Techniques: Concentration Assay

Journal: eLife

Article Title: Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication

doi: 10.7554/eLife.86067

Figure Lengend Snippet: Live-cell microscopy revealed the presence of filamentous structures extending from the surface of T. vaginalis .

Article Snippet: T. vaginalis strains G3 (ATCC PRA-98; Beckenham, UK), B7RC2 (ATCC 50167; Greenville, NC, USA), and CDC1132 (MSA1132; Mt.

Techniques:

Journal: eLife

Article Title: Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication

doi: 10.7554/eLife.86067

Figure Lengend Snippet: Different T. vaginalis strains release EVs that have specific protein content and affect the formation of filopodia and cytonemes of recipient parasites. The communication among parasites from different strains affects their attachment to host cells, most likely as a result of the increased formation in filopodia and cytonemes structures.

Article Snippet: T. vaginalis strains G3 (ATCC PRA-98; Beckenham, UK), B7RC2 (ATCC 50167; Greenville, NC, USA), and CDC1132 (MSA1132; Mt.

Techniques:

Journal: Pharmaceuticals

Article Title: Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis -Induced Cell Detachment

doi: 10.3390/ph15111350

Figure Lengend Snippet: The prior adhesion of L. gasseri bacterial cells delays Tv G3-induced alterations to the F-actin network, cell-to-cell contacts, and focal adhesion contacts. In ( A ), representative differential interference contrast micrographs of T. vaginalis -exposed cells precolonized with L. gasseri ATCC 9857 or L. gasseri KS 120.1 cells. In ( B ), percentages of cells displaying rounding and alteration to cell-to-cell contacts (12 h post-infection). In ( C ), confocal laser scanning microscopy (CLSM) images of the F-actin network. In ( D ), decrease in F-actin relative immunofluorescence intensity (RFI) over time. In ( E ), representative CLSM micrographs of vinculin-positive FAs. In ( F ), decrease in vinculin RFI/cell over time. In ( G ), decrease in the number of vinculin-positive FAs/cell over time. The micrographs shown are representative of three independent experiments. Ctl: Control. Each value shown is the mean ± SD of three independent experiments. In ( D , F , G ), * p < 0.01 for comparisons with Tv G3.

Article Snippet: We used the metronidazole-sensitive T. vaginalis reference strain G3 (ATCC PRA-98TM; Taxonomy ID: 412133) ( Tv G3), the genome of which has already been sequenced [ ].

Techniques: Infection, Confocal Laser Scanning Microscopy, Immunofluorescence, Control